Edelweiss M I, Trachtenberg A, Pinheiro E X, da-Silva J, Riegel M, Lizardo-Daudt H M, Mattevi M S
Serviço de Anatomia Patológica, Hospital de Clínicas de Porto Alegre, RS, Brasil.
Braz J Med Biol Res. 1995 Jun;28(6):679-83.
The clastogenic effect of the drug cis-diamminedichloroplatinum II (cisplatin, CDDP) was investigated in Wistar rat bone marrow cells. Male rats, 3 per treatment time, aged 4 months and weighing 250-350 g were injected intraperitoneally with 6.0 mg/kg CDDP solution, and the control group received isotonic saline. The animals were sacrificed 6, 12, 18, 24 and 48 h after the injection. The chromosome preparation was obtained from bone marrow cells. Chromatid and chromosome aberrations were investigated in 300 metaphases per animal. A significant increase in number of chromosome aberrations was observed from 6 to 24 h, the majority being of the break and gap type. After 48 h a progressive reduction was observed, without differences from the negative control. These data confirm the mutagenic effect of CDDP in rats demonstrated for mice bone marrow by micronuclei assay, for murine ovary cells and mice spermatocytes.
研究了顺二氯二氨铂（顺铂，CDDP）对Wistar大鼠骨髓细胞的致断裂效应。选用4月龄、体重250 - 350 g的雄性大鼠，每组3只，腹腔注射6.0 mg/kg CDDP溶液，对照组注射等渗盐水。注射后6、12、18、24和48小时处死动物，取骨髓细胞制备染色体。每只动物观察300个中期分裂相，研究染色单体和染色体畸变情况。6至24小时观察到染色体畸变数量显著增加，多数为断裂和裂隙型。48小时后观察到畸变数量逐渐减少，与阴性对照组无差异。这些数据证实了CDDP对大鼠的诱变作用，此前通过微核试验已在小鼠骨髓、小鼠卵巢细胞和小鼠精母细胞中得到证实。
