Yamashita S, Furuno K, Kawasaki H, Gomita Y, Yoshinaga H, Yamatogi Y, Ohtahara S
Department of Hospital Pharmacy, Okayama University Medical School, Japan.
J Chromatogr B Biomed Appl. 1995 Aug 18;670(2):354-7. doi: 10.1016/0378-4347(95)00170-0.
A simple and rapid method for the quantitation of concentrations of lamotrigine, a novel antiepileptic, in human serum was developed with high-performance liquid chromatography, using a solid-phase extraction technique. The mobile phase was composed of acetonitrile-10 mM phosphate buffer (pH 3.5) containing 5 mM sodium octanesulphonate (27:73, v/v), and components were detected at 265 nm. Retention times of acetanilide as an internal standard and lamotrigine were 3.4 and 10.3 min, respectively. The coefficients of variation were 3.1-4.5% and 4.4-9.8% for the within-day and between-day precision estimates, respectively. The extraction recovery of lamotrigine added to blank serum was 86-107%. The quantitation limit of lamotrigine was ca. 0.2 microgram/ml in 100 microliters of serum. These results suggest that the method employed in this study is useful for the routine monitoring of serum concentrations of lamotrigine in epileptic patients.
采用高效液相色谱法并结合固相萃取技术，开发了一种简单快速的定量测定人血清中新型抗癫痫药物拉莫三嗪浓度的方法。流动相由含5 mM辛烷磺酸钠的乙腈-10 mM磷酸盐缓冲液（pH 3.5）（27:73，v/v）组成，各成分在265 nm处检测。内标物乙酰苯胺和拉莫三嗪的保留时间分别为3.4分钟和10.3分钟。日内精密度估计的变异系数分别为3.1 - 4.5%，日间精密度估计的变异系数分别为4.4 - 9.8%。添加到空白血清中的拉莫三嗪的萃取回收率为86 - 107%。在100微升血清中，拉莫三嗪的定量限约为0.2微克/毫升。这些结果表明，本研究中采用的方法可用于癫痫患者血清中拉莫三嗪浓度的常规监测。
