Robertson H, Wheeler J, Thompson V, Johnson J S, Kirby J A, Morley A R
Department of Microbiology, Medical School, University of Newcastle upon Tyne, UK.
Histochem Cell Biol. 1995 Oct;104(4):331-4. doi: 10.1007/BF01464329.
A double immunohistochemical labelling procedure in paraffin-embedded renal tissue is reported in which CD3 was targeted as a T cell marker and Ki67 as a marker of cell proliferation. Proliferating and quiescent T cells were unequivocally identified in situ, and their precise location within the kidney was clarified by the use of periodic acid-Schiff counterstaining to outline the basement membranes. Proliferating tubular epithelial cells were also clearly identified. The results showed that T lymphocytes proliferate within the tubular compartment during acute renal allograft rejection. Preliminary evaluation of the method in routine transplant biopsies indicated significant correlations between histologically defined rejection grade and mean intratubular T lymphocytes per tubular cross section and between proliferation of tubular epithelial cells and of intratubular T lymphocytes. The associated tubular epithelial cell proliferation may be a response to local damage.
本文报道了一种在石蜡包埋肾组织中进行双重免疫组织化学标记的方法，其中将CD3作为T细胞标志物，Ki67作为细胞增殖标志物。原位明确鉴定出增殖和静止的T细胞，并通过使用过碘酸-希夫复染来勾勒基底膜，从而明确它们在肾脏内的精确位置。增殖的肾小管上皮细胞也被清晰地识别出来。结果表明，在急性肾移植排斥反应期间，T淋巴细胞在肾小管区域内增殖。对该方法在常规移植活检中的初步评估表明，组织学定义的排斥等级与每个肾小管横截面上的平均肾小管内T淋巴细胞数量之间，以及肾小管上皮细胞与肾小管内T淋巴细胞的增殖之间存在显著相关性。相关的肾小管上皮细胞增殖可能是对局部损伤的一种反应。
