与组蛋白mRNA 3'末端结合的多核糖体蛋白可在组蛋白前体mRNA加工中发挥作用。
The polyribosomal protein bound to the 3' end of histone mRNA can function in histone pre-mRNA processing.
作者信息
Dominski Z, Sumerel J, Hanson R J, Marzluff W F
机构信息
Department of Biochemistry, University of North Carolina, Chapel Hill 27599, USA.
出版信息
RNA. 1995 Nov;1(9):915-23.
Cell cycle-regulated histone mRNAs end in a conserved 26-nt sequence that can form a stem-loop with a six-base stem and a four-base loop. The 3' end of histone mRNA has distinct functions in the nucleus and in the cytoplasm. In the nucleus it functions in pre-mRNA processing and transport, whereas in the cytoplasm it functions in translation and regulation of histone mRNA stability. The stem-loop binding protein (SLBP), present in both nuclei and polyribosomes, is likely the trans-acting factor that binds to the 3' end of mature histone mRNA and mediates its function. A nuclear extract that efficiently processes histone pre-mRNA was prepared from mouse myeloma cells. The factor(s) that bind to the 3' end of histone mRNA can be depleted from this extract using a biotinylated oligonucleotide containing the conserved stem-loop sequence. Using this depleted extract which is deficient in histone pre-mRNA processing, we show that SLBP found in polyribosomes can restore processing, suggesting that SLBP associates with histone pre-mRNA in the nucleus, participates in processing, and then accompanies the mature mRNA to the cytoplasm.
细胞周期调控的组蛋白mRNA以一个保守的26个核苷酸的序列结尾，该序列可形成一个具有六碱基茎和四碱基环的茎环结构。组蛋白mRNA的3'端在细胞核和细胞质中具有不同的功能。在细胞核中，它在mRNA前体加工和转运中发挥作用，而在细胞质中，它在翻译和组蛋白mRNA稳定性调控中发挥作用。同时存在于细胞核和多核糖体中的茎环结合蛋白（SLBP）可能是与成熟组蛋白mRNA的3'端结合并介导其功能的反式作用因子。从小鼠骨髓瘤细胞中制备了一种能有效加工组蛋白mRNA前体的核提取物。使用含有保守茎环序列的生物素化寡核苷酸可从该提取物中去除与组蛋白mRNA 3'端结合的因子。利用这种缺乏组蛋白mRNA前体加工能力的耗尽提取物，我们发现多核糖体中的SLBP可以恢复加工过程，这表明SLBP在细胞核中与组蛋白mRNA前体结合，参与加工，然后伴随成熟mRNA进入细胞质。
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