D'Alessandri L, Ranscht B, Winterhalter K H, Vaughan L
Laboratorium für Biochemie I, ETH-Zentrum, Zürich, Switzerland.
Curr Eye Res. 1995 Oct;14(10):911-26. doi: 10.3109/02713689508995131.
The neural immunoglobulin-like cell adhesion molecule contactin/F11 and the extracellular matrix glycoprotein tenascin-C are prominent molecules in the developing nervous system which interact in in vitro assays (Zisch et al., J. Cell Biol. 119, 203-213). To determine their potential role in neural development, the distribution of tenascin-C and contactin/F11 was examined in the developing chick retina. The onset of both tenascin-C and contactin/F11 expression coincides with the appearance of ganglion cell dendrides and neurites from bipolar and amacrine cells in the inner layer (IPL) at E8, and the extension of bipolar and horizontal cell processes in the outer plexiform layer (OPL) at E9. Contactin/F11 expression is co-ordinately upregulated with the TN190 and TN200 tenascin-C isoforms between embryonic day 8 (E8) and E17, while little, if any, of the TN220 isoform, which does not bind contactin/F11, is detected. In situ hybridization reveals that tenascin-C and contactin/F11 mRNAs are synthesized by different neuronal types. Tenascin-C mRNA probes hybridize to amacrine and displaced amacrine neurons, and horizontal neurons. In cultured retinal cells, tenascin-C is also present on process-bearing neurofilament-positive cells. Contactin/F11 mRNA is detected in bipolar cells or their precursors from E8-9, and later in horizontal and ganglion neurons. The highest levels and greatest overlap in the synaptic IPL and OPL are reached at E17, when the stratification of the retina is nearly complete. These results are consistent with a putative role for contactin/F11-tenascin-C interactions in the establishment of synaptic layers in the retina.
神经免疫球蛋白样细胞黏附分子接触蛋白/F11和细胞外基质糖蛋白腱生蛋白-C是发育中的神经系统中的重要分子，它们在体外试验中相互作用（齐施等人，《细胞生物学杂志》119卷，203 - 213页）。为了确定它们在神经发育中的潜在作用，研究了腱生蛋白-C和接触蛋白/F11在发育中的鸡视网膜中的分布。腱生蛋白-C和接触蛋白/F11的表达起始与E8时内层（内网层）中神经节细胞树突以及双极细胞和无长突细胞的神经突的出现，以及E9时外网状层中双极细胞和水平细胞突起的延伸同时发生。在胚胎第8天（E8）至E17期间，接触蛋白/F11的表达与腱生蛋白-C的TN190和TN200亚型协同上调，而几乎检测不到不与接触蛋白/F11结合的TN220亚型。原位杂交显示，腱生蛋白-C和接触蛋白/F11的mRNA由不同的神经元类型合成。腱生蛋白-C mRNA探针与无长突细胞、移位无长突神经元和水平神经元杂交。在培养的视网膜细胞中，腱生蛋白-C也存在于带有突起的神经丝阳性细胞上。在E8 - 9时，在双极细胞或其前体细胞中检测到接触蛋白/F11 mRNA，随后在水平神经元和神经节神经元中检测到。在E17时，当视网膜分层几乎完成时，在突触内网层和外网层达到最高水平和最大重叠。这些结果与接触蛋白/F11 - 腱生蛋白-C相互作用在视网膜突触层建立中的假定作用一致。
