Idänpään-Heikkilä J J, Rauhala P, Männistö P T
Department of Pharmacology and Toxicology, University of Helsinki, Finland.
Eur J Pharmacol. 1995 Sep 15;284(1-2):227-30. doi: 10.1016/0014-2999(95)00462-t.
Effects of i.c.v. pretreatment with antisense oligodeoxynucleotides (antisense oligos) targeted against either mu- or delta-opioid receptors on morphine-induced release of growth hormone (GH) and prolactin were studied in male rats. The stimulation of GH secretion by i.c.v. morphine was completely inhibited by the antisense oligo targeted against the cloned mu-opioid receptor and significantly reduced by the antisense oligo targeted against the cloned delta-opioid receptor. The antisense oligo targeted against the cloned mu-opioid receptor, but not that targeted against the cloned delta-opioid receptor, abolished the stimulatory effect of acute morphine on prolactin secretion. It is concluded that both the GH and prolactin secretion stimulating effect of morphine is mainly mediated by the cloned mu-opioid receptor. Further, the cloned delta-opioid receptor is involved in the morphine-induced stimulation of GH secretion.
在雄性大鼠中，研究了脑室内（i.c.v.）预先注射针对μ-或δ-阿片受体的反义寡脱氧核苷酸（反义寡核苷酸）对吗啡诱导的生长激素（GH）和催乳素释放的影响。针对克隆的μ-阿片受体的反义寡核苷酸完全抑制了脑室内注射吗啡对GH分泌的刺激作用，而针对克隆的δ-阿片受体的反义寡核苷酸则显著降低了该刺激作用。针对克隆的μ-阿片受体的反义寡核苷酸消除了急性吗啡对催乳素分泌的刺激作用，而针对克隆的δ-阿片受体的反义寡核苷酸则没有此作用。得出的结论是，吗啡对GH和催乳素分泌的刺激作用主要由克隆的μ-阿片受体介导。此外，克隆的δ-阿片受体参与了吗啡诱导的GH分泌刺激。
