Escott K J, Beattie D T, Connor H E, Brain S D
Pharmacology Group, King's College, London, UK.
Eur J Pharmacol. 1995 Sep 15;284(1-2):69-76. doi: 10.1016/0014-2999(95)00367-t.
Electrical stimulation of the trigeminal ganglion causes an increase in facial skin blood flow in the anaesthetised rat, as measured by laser Doppler flowmetry. We investigated the modulation of this neurogenic vasodilator response using selective receptor agonists for putative prejunctional inhibitory receptors, as well as other pharmacological agents to further characterise this response. [D-Ala2,Me-Phe4,Gly5-ol]enkephalin (DAGO, a mu-opioid receptor agonist) inhibited the vasodilator response in a dose-related (0.058-5.8 mumol/kg i.v.) and naloxone-sensitive manner. A similar inhibitory response was observed with the local anaesthetic lignocaine (2% w/v, s.c. 20 microliters). In contrast, the histamine H3-receptor agonist alpha-methylhistamine (15 or 35 mumol/kg, i.v.) and the 5-HT1D receptor agonists sumatriptan (0.24 or 2.4 mumol/kg, i.v.) and CP 122,288 (0.0003-3 mumol/kg, i.v.) had no effect on these responses. Similarly, atropine (1.5 mumol/kg, i.v.) and indomethacin (28 mumol/kg, i.v.) did not alter the vasodilatation observed in this model. In conclusion, only mu-opioid receptor activation and local anaesthetic had any inhibitory action on the neurogenic vasodilatation observed in this model.
通过激光多普勒血流仪测量发现，电刺激麻醉大鼠的三叉神经节会导致面部皮肤血流增加。我们使用针对假定的突触前抑制性受体的选择性受体激动剂以及其他药理试剂来研究这种神经源性血管舒张反应的调节，以进一步表征这种反应。[D-丙氨酸2，甲基苯丙氨酸4，甘氨酸5-醇]脑啡肽（DAGO，一种μ-阿片受体激动剂）以剂量相关（0.058-5.8 μmol/kg静脉注射）且对纳洛酮敏感的方式抑制血管舒张反应。局部麻醉药利多卡因（2% w/v，皮下注射20微升）也观察到类似的抑制反应。相比之下，组胺H3受体激动剂α-甲基组胺（15或35 μmol/kg，静脉注射）以及5-HT1D受体激动剂舒马曲坦（0.24或2.4 μmol/kg，静脉注射）和CP 122,288（0.0003-3 μmol/kg，静脉注射）对这些反应没有影响。同样，阿托品（1.5 μmol/kg，静脉注射）和吲哚美辛（28 μmol/kg，静脉注射）也没有改变该模型中观察到的血管舒张。总之，在该模型中，只有μ-阿片受体激活和局部麻醉药对神经源性血管舒张有任何抑制作用。
