在景天酸代谢（CAM）兼性植物长寿花（Kalanchoe blossfeldiana Poelln. cv. Tom Thumb）叶片中多个磷酸烯醇式丙酮酸羧化酶（PEPC）基因的鉴定。
Identification of multiple PEPC isogenes in leaves of the facultative Crassulacean acid metabolism (CAM) plant Kalanchoe blossfeldiana Poelln. cv. Tom Thumb.
作者信息
Gehrig H, Taybi T, Kluge M, Brulfert J
机构信息
Institut für Botanik, Technischen Hochschule Darnstadt, Germany.
出版信息
FEBS Lett. 1995 Dec 27;377(3):399-402. doi: 10.1016/0014-5793(95)01397-0.
In the facultative Crassulacean Acid Metabolism (CAM) plant Kulanchoe blossfeldiana cv. Tom Thumb, CAM can be induced by short-day treatment or water deficiency stress. From young leaves of well-watered and water-stressed individuals of this plant, cDNA clones coding for a partial sequence of the key enzyme of CAM, phosphoenolpyruvate carboxylase, were isolated after transcription of mRNA. cDNA polymorphism was established by enzyme restriction profiles and sequencing data. Four PEPC isogenes could be shown to exist in K. blossfeldiana forming two gene pairs, with 95%-98% homology inside and only 75% between the pairs. One cDNA sequence pair having a length of 1113 bp and an open reading frame of 371 AA was identified as PEPC isoform specific for the C3 state, whereas the pair having a length of 1116 bp and an open reading frame of 372 AA could be attributed to the CAM state. These results were confirmed by Southern Blot hybridization.
在兼性景天酸代谢（CAM）植物长寿花品种拇指汤姆中，短日照处理或水分亏缺胁迫可诱导CAM的产生。从该植物水分充足和水分胁迫个体的幼叶中，在mRNA转录后分离出编码CAM关键酶磷酸烯醇式丙酮酸羧化酶部分序列的cDNA克隆。通过酶切图谱和测序数据确定了cDNA多态性。结果表明，长寿花中存在四个磷酸烯醇式丙酮酸羧化酶（PEPC）基因，形成两个基因对，基因对内同源性为95%-98%，而基因对之间仅为75%。一对长度为1113bp、开放阅读框为371个氨基酸的cDNA序列被鉴定为C3状态特有的PEPC同工型，而另一对长度为1116bp、开放阅读框为372个氨基酸的序列则属于CAM状态。Southern杂交证实了这些结果。
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