酵母中过氧化物酶体功能的异源互补：酿酒酵母PAS3基因可恢复多形汉逊酵母per9缺失突变体中的过氧化物酶体生物发生。
Heterologous complementation of peroxisome function in yeast: the Saccharomyces cerevisiae PAS3 gene restores peroxisome biogenesis in a Hansenula polymorpha per9 disruption mutant.
作者信息
Kiel J A, Keizer-Gunnink I K, Krause T, Komori M, Veenhuis M
机构信息
Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Haren, The Netherlands.
出版信息
FEBS Lett. 1995 Dec 27;377(3):434-8. doi: 10.1016/0014-5793(95)01385-7.
PER genes are essential for the biogenesis of peroxisomes in the yeast Hansenula polymorpha. Here we describe the functional complementation of a H. polymorpha per9 disruption strain (delta per9) by a heterologous gene. The Saccharomyces cerevisiae Pas3p, a homologue of per9p, restored peroxisome biogenesis and peroxisomal protein import in the delta per9 mutant, allowing it to grow again on methanol as sole carbon and energy source. This result shows that heterologous complementation of peroxisome function in yeast is indeed feasible and furthermore suggests that H. polymorpha delta per9 may be the candidate of choice to attempt the isolation of Per9p homologues from higher eukaryotes by functional complementation.
PER基因对于多形汉逊酵母中过氧化物酶体的生物合成至关重要。在此，我们描述了一个异源基因对多形汉逊酵母per9缺失菌株（Δper9）的功能互补作用。酿酒酵母的Pas3p是per9p的同源物，它恢复了Δper9突变体中的过氧化物酶体生物合成和过氧化物酶体蛋白导入，使其能够再次在甲醇作为唯一碳源和能源的培养基上生长。这一结果表明，酵母中过氧化物酶体功能的异源互补确实可行，此外还表明，多形汉逊酵母Δper9可能是通过功能互补从高等真核生物中分离Per9p同源物的理想候选菌株。
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