豚鼠肝脏中编码过氧化物酶体2-烯酰辅酶A水合酶/3-羟酰基辅酶A脱氢酶的两个cDNA的克隆及组织表达
Cloning and tissue expression of two cDNAs encoding the peroxisomal 2-enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase in the guinea pig liver.
作者信息
Caira F, Cherkaoui-Malki M, Hoefler G, Latruffe N
机构信息
Laboratoire de Biologie Moléculaire et Cellulaire, Université de Bourgogne, Dijon, France.
出版信息
FEBS Lett. 1996 Jan 2;378(1):57-60. doi: 10.1016/0014-5793(95)01425-x.
The 2-enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase (HD) is the second enzyme of the peroxisomal beta-oxidation pathway. In human and rat, only one HD mRNA has been so far detected in the liver. This paper reports for the first time in a mammal species, the guinea pig, the cloning and sequencing of two cDNAs encoding an HD. The 3,274 nucleotide-cDNA is a strictly identical but longer copy of the 2,494 nucleotide-form. A 2,178 bp-open reading frame encodes a protein of 726 amino acids (M(r) 79.3 kDa) with the peroxisomal-targeting signal (tripeptide SKL) at the carboxyterminus. Northern blot analysis of HD mRNA identified three mRNAs of respective sizes 3.5, 2.6 and 1.6 kb in the guinea pig liver and kidneys.
2-烯酰辅酶A水合酶/3-羟酰基辅酶A脱氢酶（HD）是过氧化物酶体β-氧化途径的第二种酶。在人和大鼠中，迄今为止在肝脏中仅检测到一种HD mRNA。本文首次报道了在一种哺乳动物豚鼠中，编码HD的两个cDNA的克隆和测序。3274个核苷酸的cDNA是2494个核苷酸形式的完全相同但更长的拷贝。一个2178 bp的开放阅读框编码一个由726个氨基酸组成的蛋白质（分子量79.3 kDa），其羧基末端带有过氧化物酶体靶向信号（三肽SKL）。对HD mRNA的Northern印迹分析在豚鼠肝脏和肾脏中鉴定出三种大小分别为3.5、2.6和1.6 kb的mRNA。
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