Hope J C, Cumberbatch M, Fielding I, Dearman R J, Kimber I, Hopkins S J
University of Manchester Rheumatic Diseases Centre, Hope Hospital, Salford, UK.
Immunology. 1995 Nov;86(3):441-7.
Skin sensitization with chemical allergens is associated with the activation and proliferation of T lymphocytes within lymph nodes draining the site of exposure. These events are accompanied by the secretion of interleukin-6 (IL-6) by lymph node cells (LNC). We have investigated the cellular source of IL-6 seventy-two hours following primary exposure of mice to the contact allergen oxazolone. Immunocytochemical analyses of sections of activated lymph nodes have revealed that cells expressing IL-6 are located within the T-dependent lymph node paracortex, with none present in lymphoid follicles. Cells which expressed IL-6 cofractionated exclusively with LNC of low buoyant density, the majority of which also expressed membrane Ia and had a dendritic morphology. Depletion of dendritic cells from LNC culture was associated with a significant decrease in the secretion of IL-6 by the residual population. These data demonstrate that dendritic cells are a major source of IL-6 within lymph nodes during primary immune responses to cutaneous antigens.
化学过敏原引起的皮肤致敏与暴露部位引流淋巴结内T淋巴细胞的激活和增殖有关。这些事件伴随着淋巴结细胞（LNC）分泌白细胞介素-6（IL-6）。我们研究了小鼠初次接触接触性变应原恶唑酮72小时后IL-6的细胞来源。对活化淋巴结切片的免疫细胞化学分析显示，表达IL-6的细胞位于T细胞依赖的淋巴结副皮质区，淋巴滤泡中没有。表达IL-6的细胞仅与低浮力密度的LNC共分离，其中大多数细胞也表达膜Ia且具有树突状形态。从LNC培养物中去除树突状细胞与剩余细胞群分泌IL-6的显著减少有关。这些数据表明，在对皮肤抗原的初次免疫反应中，树突状细胞是淋巴结内IL-6的主要来源。
