Rascle Philippe, Jacquelin Béatrice, Petitdemange Caroline, Contreras Vanessa, Planchais Cyril, Lazzerini Marie, Dereuddre-Bosquet Nathalie, Le Grand Roger, Mouquet Hugo, Huot Nicolas, Müller-Trutwin Michaela
Institut Pasteur, HIV Inflammation and Persistence Unit, 28 rue du Dr Roux, 75724 Paris Cedex 15, France.
Université Paris Diderot, Sorbonne Paris Cité, Paris, France.
iScience. 2021 Sep 9;24(10):103109. doi: 10.1016/j.isci.2021.103109. eCollection 2021 Oct 22.
B cell follicles (BCFs) in lymph nodes (LNs) are generally exempt of CD8 T and NK cells. African green monkeys (AGMs), a natural host of simian immunodeficiency virus (SIV), display NK cell-mediated viral control in BCF. NK cell migration into BCF in chronically SIVagm-infected AGM is associated with CXCR5 NK cells. We aimed to identify the mechanism leading to CXCR5 expression on NK cells. We show that CXCR5 NK cells in LN were induced following SIVagm infection. CXCR5 NK cells accumulated preferentially in BCF with proliferating B cells. Autologous NK-B cell co-cultures in transwell chambers induced CXCR5 NK cells. Transcriptome analysis of CXCR5 NK cells revealed expression of and . IL-6 induced CXCR5 on AGM and human NK cells. mRNA was detected in LN at higher levels during SIVagm than SIVmac infection and often produced by plasma cells. Our study reveals a mechanism of B cell-dependent NK cell regulation.
淋巴结中的B细胞滤泡通常不存在CD8 T细胞和NK细胞。非洲绿猴是猿猴免疫缺陷病毒（SIV）的天然宿主，在B细胞滤泡中表现出NK细胞介导的病毒控制作用。在慢性感染SIVagm的非洲绿猴中，NK细胞迁移到B细胞滤泡与CXCR5 NK细胞有关。我们旨在确定导致NK细胞上CXCR5表达的机制。我们发现，SIVagm感染后，淋巴结中的CXCR5 NK细胞被诱导产生。CXCR5 NK细胞优先在B细胞滤泡中与增殖的B细胞一起积累。在Transwell小室中进行的自体NK细胞与B细胞共培养可诱导产生CXCR5 NK细胞。对CXCR5 NK细胞的转录组分析揭示了 和 的表达。IL-6可诱导非洲绿猴和人类NK细胞上的CXCR5表达。在SIVagm感染期间，淋巴结中 mRNA的检测水平高于SIVmac感染，且通常由浆细胞产生。我们的研究揭示了一种B细胞依赖性NK细胞调节机制。
