Chandrashekar R, Van Swinderen B, Taylor H R, Weil G J
Department of Internal Medicine, Washington University School of Medicine, St Louis, MS 63110, USA.
Int J Parasitol. 1995 Aug;25(8):983-8. doi: 10.1016/0020-7519(95)00011-p.
Antibody responses to recombinant Onchocerca volvulus antigens were studied in experimentally infected chimpanzees. Sera from 3 groups of 6 animals were tested by ELISA with recombinant antigens OC 3.6 and OC 9.3. Groups I and II were treated with 200 micrograms/kg of ivermectin on the day of infection or on day 28, respectively. Group III were untreated controls. Antibodies to OC 3.6 developed during the prepatent period in all 3 groups. In contrast, antibodies to OC 9.3 were usually first detected around the time of onset of patency. Several animals had early antibody responses to OC 9.3, but these animals subsequently failed to develop microfilarial patency. Only 1 of 6 animals in group I produced a strong antibody response to OC 9.3 while all 12 animals in groups II and III developed antibodies to this antigen. Although there was some inconsistency in antibody responses observed in each treatment group, the results suggest that OC 9.3 may be more useful than OC 3.6 for monitoring the efficacy of prophylactic drugs or vaccines for onchocerciasis while OC 3.6 may be useful for detecting exposure to the parasite and early infection, regardless of the later outcome of the infection.
在实验感染的黑猩猩中研究了对重组盘尾丝虫抗原的抗体反应。用重组抗原OC 3.6和OC 9.3通过ELISA检测了3组每组6只动物的血清。第一组和第二组分别在感染当天或第28天用200微克/千克的伊维菌素进行治疗。第三组为未治疗的对照组。所有3组在潜伏期均产生了针对OC 3.6的抗体。相比之下，针对OC 9.3的抗体通常在成虫出现时首次检测到。几只动物对OC 9.3有早期抗体反应，但这些动物随后未能出现微丝蚴血症。第一组6只动物中只有1只对OC 9.3产生了强烈的抗体反应，而第二组和第三组的所有12只动物都产生了针对该抗原的抗体。尽管在每个治疗组中观察到的抗体反应存在一些不一致，但结果表明，对于监测盘尾丝虫病预防药物或疫苗的疗效，OC 9.3可能比OC 3.6更有用，而OC 3.6可能有助于检测寄生虫暴露和早期感染，无论感染的后期结果如何。
