来自两个硫化叶菌属物种的编码NAD依赖性乙醇脱氢酶的基因在大肠杆菌中的克隆与过表达。
Cloning and overexpression in Escherichia coli of the genes encoding NAD-dependent alcohol dehydrogenase from two Sulfolobus species.
作者信息
Cannio R, Fiorentino G, Carpinelli P, Rossi M, Bartolucci S
机构信息
Dipartimento di Chimica Organica e Biologica, Università degli Studi di Napoli Federico II, Italy.
出版信息
J Bacteriol. 1996 Jan;178(1):301-5. doi: 10.1128/jb.178.1.301-305.1996.
Abstract
The gene adh encoding a NAD-dependent alcohol dehydrogenase from the novel strain RC3 of Sulfolobus sp. was cloned and sequenced. Both the adh gene from Sulfolobus sp. strain RC3 and the alcohol dehydrogenase gene from Sulfolobus solfataricus (DSM 1617) were expressed at a high level in Escherichia coli, and the recombinant enzymes were purified, characterized, and compared. Only a few amino acid replacements were responsible for the different kinetic and physicochemical features investigated.
摘要
克隆并测序了来自嗜硫裂片菌新菌株RC3的编码NAD依赖性乙醇脱氢酶的adh基因。嗜硫裂片菌菌株RC3的adh基因和嗜热栖热菌（DSM 1617）的乙醇脱氢酶基因均在大肠杆菌中高水平表达，对重组酶进行了纯化、表征和比较。在所研究的不同动力学和物理化学特性中，只有少数氨基酸替换起作用。
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