一种新基因sanA的扩增消除了大肠杆菌中的万古霉素敏感缺陷。
Amplification of a novel gene, sanA, abolishes a vancomycin-sensitive defect in Escherichia coli.
作者信息
Rida S, Caillet J, Alix J H
机构信息
Institut de Biologie Physico-Chimique, Paris, France.
出版信息
J Bacteriol. 1996 Jan;178(1):94-102. doi: 10.1128/jb.178.1.94-102.1996.
Abstract
We have isolated an Escherichia coli gene which, when overexpressed, is able to complement the permeability defects of a vancomycin-susceptible mutant. This gene, designated sanA, is located at min 47 of the E. coli chromosome and codes for a 20-kDa protein with a highly hydrophobic amino-terminal segment. A strain carrying a null mutation of the sanA gene, transferred to the E. coli chromosome by homologous recombination, is perfectly viable, but after two generations at high temperature (43 degrees C), the barrier function of its envelope towards vancomycin is defective.
摘要
我们分离出了一个大肠杆菌基因，该基因在过表达时能够弥补对万古霉素敏感的突变体的通透性缺陷。这个名为sanA的基因位于大肠杆菌染色体的47分钟处，编码一种20 kDa的蛋白质，其氨基末端片段具有高度疏水性。通过同源重组转移到大肠杆菌染色体上的携带sanA基因无效突变的菌株完全可以存活，但在高温（43摄氏度）下培养两代后，其包膜对万古霉素的屏障功能出现缺陷。
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Gene. 1993 May 15;127(1):117-20. doi: 10.1016/0378-1119(93)90625-d.
2
A mutation in the dsbA gene coding for periplasmic disulfide oxidoreductase reduces transcription of the Escherichia coli ompF gene.
Mol Gen Genet. 1993 Mar;237(3):407-11. doi: 10.1007/BF00279445.
3
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4
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Trends Biochem Sci. 1993 Jan;18(1):7-12. doi: 10.1016/0968-0004(93)90080-7.
5
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Mol Microbiol. 1993 Jan;7(1):141-50. doi: 10.1111/j.1365-2958.1993.tb01105.x.
6
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7
OmpF assembly mutants of Escherichia coli K-12: isolation, characterization, and suppressor analysis.大肠杆菌K-12的OmpF组装突变体：分离、表征及抑制子分析。
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Genes Dev. 1993 Dec;7(12B):2618-28. doi: 10.1101/gad.7.12b.2618.
10
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