Preston A, Maskell D, Johnson A, Moxon E R
Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, United Kingdom.
J Bacteriol. 1996 Jan;178(2):396-402. doi: 10.1128/jb.178.2.396-402.1996.
The 169 phenotype of Haemophilus influenzae results from a mutation leading to a lipopolysaccharide molecule consisting only of lipid A and a single phosphorylated 2-keto-3-deoxyoctulosonic acid residue. In this paper we describe the identification of a gene which, when mutated, results in the 169 phenotype. We have named the gene isn. The predicted amino acid sequence of Isn is homologous to the product of the lmbN gene involved in the biosynthesis of the sugar-containing antibiotic lincomycin by Streptomyces lincolnensis. lsn is situated between two loci that are homologous to the dpp and art periplasmic permease systems in Escherichia coli. Northern (RNA) blot and primer extension analyses reveal that isn is transcribed as a monocistronic mRNA. Potential functions of Isn protein are discussed.
流感嗜血杆菌的169表型是由一个突变导致的，该突变产生一种仅由脂质A和单个磷酸化的2-酮-3-脱氧辛酸残基组成的脂多糖分子。在本文中，我们描述了一个基因的鉴定，该基因发生突变时会导致169表型。我们将该基因命名为isn。Isn的预测氨基酸序列与林肯链霉菌参与含糖类抗生素林可霉素生物合成的lmbN基因的产物同源。Isn位于与大肠杆菌中的dpp和art周质通透酶系统同源的两个基因座之间。Northern（RNA）印迹和引物延伸分析表明，isn转录为单顺反子mRNA。文中还讨论了Isn蛋白的潜在功能。
