Shen Z, Byers D M
Department of Pediatrics, Atlantic Resarch Centre, Dalhousie University, Halifax, Nova Scotia, Canada.
J Bacteriol. 1996 Jan;178(2):571-3. doi: 10.1128/jb.178.2.571-573.1996.
We report the isolation of Vibrio harveyi acyl carrier protein (ACP) and cloning of a 3,973-bp region containing the fabG (encoding 3-ketoacyl-ACP reductase, 25.5 kDa), acpP (encoding ACP, 8.7 kDa), fabF (encoding 3-ketoacyl-ACP synthase II, 43.1 kDa), and pabC (encoding aminodeoxychorismate lyase, 29.9 kDa) genes. Predicted amino acid sequences were, respectively, 78, 86, 76, and 35% identical to those of the corresponding Escherichia coli proteins. Five of the 11 sequence differences between V. harveyi and E. coli ACP were nonconservative amino acid differences concentrated in a loop region between helices I and II.
我们报道了哈维氏弧菌酰基载体蛋白(ACP)的分离以及一个3973 bp区域的克隆,该区域包含fabG(编码3-酮酰基-ACP还原酶,25.5 kDa)、acpP(编码ACP,8.7 kDa)、fabF(编码3-酮酰基-ACP合酶II,43.1 kDa)和pabC(编码氨基脱氧分支酸裂解酶,29.9 kDa)基因。预测的氨基酸序列与相应的大肠杆菌蛋白的氨基酸序列分别有78%、86%、76%和35%的同一性。哈维氏弧菌和大肠杆菌ACP之间11个序列差异中的5个是非保守氨基酸差异,集中在螺旋I和螺旋II之间的一个环区域。