在大肠杆菌K-12中过量表达通用应激蛋白UspA的影响。
Effects of overproducing the universal stress protein, UspA, in Escherichia coli K-12.
作者信息
Nyström T, Neidhardt F C
机构信息
Department of General and Marine Microbiology, Lundberg Laboratory, University of Göteborg, Sweden.
出版信息
J Bacteriol. 1996 Feb;178(3):927-30. doi: 10.1128/jb.178.3.927-930.1996.
Abstract
A plasmid with the structural uspA gene under the control of a tac promoter was used to study the effects of altering uspA expression levels under various growth conditions. We found that increasing UspA synthesis to levels corresponding to physiologically induced levels decreased the cell growth rate in minimal medium and reduced or abolished the cells' capacity to adapt to upshift conditions. As was demonstrated by two-dimensional gel electrophoresis, increased uspA expression caused global changes in the pattern of protein synthesis. In addition, electrophoretic analysis together with V8 protease peptide mapping demonstrated that the pIs of some specific proteins became more acidic as a result of the elevation of the levels of UspA.
摘要
使用一个在tac启动子控制下含有结构uspA基因的质粒，来研究在各种生长条件下改变uspA表达水平的影响。我们发现，将UspA合成增加到与生理诱导水平相对应的水平，会降低基本培养基中的细胞生长速率，并降低或消除细胞适应向上变化条件的能力。二维凝胶电泳表明，uspA表达增加导致蛋白质合成模式发生全局性变化。此外，电泳分析和V8蛋白酶肽图谱分析表明，由于UspA水平升高，一些特定蛋白质的等电点变得更偏酸性。
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本文引用的文献
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Isolation and properties of a mutant of Escherichia coli with an insertional inactivation of the uspA gene, which encodes a universal stress protein.编码一种通用应激蛋白的uspA基因发生插入失活的大肠杆菌突变体的分离与特性
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