Cerra M, Cecco L, Montella M, Tuccillo F, Bonelli P, Botti G
Biochemistry Department, National Cancer Institute Fondazione G. Pascale, Napoli, Italy.
Int J Biol Markers. 1995 Jul-Sep;10(3):136-42. doi: 10.1177/172460089501000302.
The relationship between epidermal growth factor receptor (EGFR) status and various prognostic factors was investigated in 70 human breast cancer specimens. Epidermal growth factor receptor was determined by radioligand binding assay, standardized by the EORTC Receptor Study Group, using hydroxyapatite to separate receptor-bound and free ligand. The percentage of EGFR positivity was 80% when the cutoff was set at 5 fmol/mg of membrane protein; this percentage was among the highest hitherto reported. Regression analysis of EGFR versus ER and PR levels confirmed an inverse relationship between EGFR and ER (p = 0.022) as well as between EGFR and PR (p = 0.024). Univariate analysis of the EGFR data stratified according to steroid hormone receptor status showed EGFR to be negatively associated with ER and PR. No association was found between EGFR and menopausal status, axillary lymph node involvement, tumor size, and differentiation grade. A direct association between EGFR status and Ki-67 positive cell rate could be demonstrated.
在70例人类乳腺癌标本中研究了表皮生长因子受体（EGFR）状态与各种预后因素之间的关系。表皮生长因子受体通过放射性配体结合试验测定，该试验由欧洲癌症研究与治疗组织（EORTC）受体研究组标准化，使用羟基磷灰石分离受体结合的配体和游离配体。当将临界值设定为5 fmol/mg膜蛋白时，EGFR阳性率为80%；该百分比是迄今报道的最高百分比之一。EGFR与雌激素受体（ER）和孕激素受体（PR）水平的回归分析证实，EGFR与ER之间存在负相关（p = 0.022），EGFR与PR之间也存在负相关（p = 0.024）。根据类固醇激素受体状态对EGFR数据进行单因素分析，结果显示EGFR与ER和PR呈负相关。未发现EGFR与绝经状态、腋窝淋巴结受累、肿瘤大小及分化程度之间存在关联。EGFR状态与Ki-67阳性细胞率之间存在直接关联。
