Mason J D, Hehar S S, Holden M, Jones N S
Department of Otorhinolaryngology, Leicester Royal Infirmary.
J Laryngol Otol. 1995 Dec;109(12):1181-3. doi: 10.1017/s0022215100132372.
A reproducible method for the estimation of volumes of small tissue samples was needed to allow comparison of the biological activity of tissue explants and to relate symptom improvement to procedures such as adenoidectomy or polypectomy. Holden's apparatus, which utilizes fluid displacement between two chambers, was compared to displacement in a 20 ml syringe. Two observers made four readings each on 20 tissue samples (range 0.37 to 4.70 ml) using both methods for comparison. A two-way analysis of variance for replicated data gave an F ratio of 0.6885 (df = 19; p > 0.8) for Holden's apparatus, and an F ratio of 1.4307 (df = 19; p > 0.1) for the 20 ml syringe. The standard error of the mean for repeated readings on the same sample by an observer was 0.001 ml for Holden's apparatus and 0.014 ml for the syringe method. For accurate estimation of small tissue volumes within 0.01 ml we recommend Holden's apparatus.
为了能够比较组织外植体的生物活性，并将症状改善与诸如腺样体切除术或息肉切除术等手术相关联，需要一种可重复的方法来估计小组织样本的体积。将利用两个腔室之间的液体置换的霍尔登装置与20毫升注射器中的置换进行了比较。两名观察者使用这两种方法对20个组织样本（范围为0.37至4.70毫升）各进行了四次读数以作比较。对重复数据进行的双向方差分析得出，霍尔登装置的F值为0.6885（自由度=19；p>0.8），20毫升注射器的F值为1.4307（自由度=19；p>0.1）。观察者对同一样本进行重复读数的平均标准误差，霍尔登装置为0.001毫升，注射器法为0.014毫升。为了精确估计0.01毫升以内的小组织体积，我们推荐使用霍尔登装置。
