Klimyuk V I, Nussaume L, Harrison K, Jones J D
Sainsbury Laboratory, John Innes Institute, Norwich, United Kingdom.
Mol Gen Genet. 1995 Dec 10;249(4):357-65. doi: 10.1007/BF00287097.
Enhancer trap derivatives of the maize Dissociation (Ds) transposon were introduced into Arabidopsis thaliana. The enhancer trap Ds was so designed that upon transposition to sites containing regulatory sequences in adjacent genomic DNA, transcription of a Ds-borne beta-glucuronidase (GUS) gene would be activated. Sixty percent of all transposition events were associated with GUS expression patterns including one linked to a mutant phenotype. Patterns of GUS expression were found in various organs and were stably inheritable in the F4 and F5 progenies. These results demonstrate the potential value of the technique as a means for detection of developmentally regulated genes and analysis of their function. The enhancer trap construct used in our experiments, as well as the seeds of primary transformants are publicly available.
玉米解离（Ds）转座子的增强子捕获衍生物被导入拟南芥中。增强子捕获Ds的设计使得在转座到相邻基因组DNA中含有调控序列的位点时，Ds携带的β-葡萄糖醛酸酶（GUS）基因的转录会被激活。所有转座事件的60%与GUS表达模式相关，其中一种与突变表型有关。在各个器官中都发现了GUS表达模式，并且在F4和F5后代中稳定遗传。这些结果证明了该技术作为检测发育调控基因及其功能分析手段的潜在价值。我们实验中使用的增强子捕获构建体以及初级转化体的种子都是公开可用的。
