Sidorenkov I N, Nosovskaia E A, Kostrov S V
Mol Biol (Mosk). 1995 May-Jun;29(3):507-11.
Gene of thermostable Bacillus stearothermophilus metalloprotease was cloned and expressed in mesophilic Bacillus subtilis cells. It was demonstrated that this gene is closely related by its restriction map to B. stearothermophilus T metalloprotease gene cloned earlier. Thermostability level and thermal optimum of activity of metalloprotease, the product of cloned gene expression, were estimated.
嗜热脂肪芽孢杆菌金属蛋白酶基因被克隆并在嗜温枯草芽孢杆菌细胞中表达。结果表明，该基因的限制性图谱与先前克隆的嗜热脂肪芽孢杆菌T金属蛋白酶基因密切相关。对克隆基因表达产物金属蛋白酶的热稳定性水平和活性最适温度进行了评估。
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