Pollack S J, Sadler I I, Hawtin S R, Tailor V J, Shearman M S
Merck Sharp and Dohme Research Laboratories, Neuroscience Research Centre, Harlow, Essex, UK.
Neurosci Lett. 1995 Sep 15;197(3):211-4. doi: 10.1016/0304-3940(95)11939-t.
We recently reported that several sulfate-containing glycosaminoglycans, a class of compounds associated with the beta-amyloid plaques of Alzheimer's disease, attenuate the toxic effects of beta-amyloid fragments beta 25-35 and beta 1-40. The amyloid-binding sulfonated dye Congo Red was shown to have a similar effect. Using two clonal cell lines, we now demonstrate that several sulfonated dyes attenuate beta-amyloid toxicity and that the protective effect appears specific for compounds whose sulfonate groups can interact with the beta-pleated structure of aggregated amyloid. These results suggest that by binding beta-amyloid these compounds may prevent toxic interactions of the peptide with cells.
我们最近报道，几种含硫酸根的糖胺聚糖（一类与阿尔茨海默病的β-淀粉样斑块相关的化合物）可减轻β-淀粉样片段β25-35和β1-40的毒性作用。已证明淀粉样蛋白结合磺化染料刚果红具有类似作用。利用两种克隆细胞系，我们现在证明几种磺化染料可减轻β-淀粉样蛋白毒性，且这种保护作用似乎对其磺酸根基团能与聚集淀粉样蛋白的β-折叠结构相互作用的化合物具有特异性。这些结果表明，通过结合β-淀粉样蛋白，这些化合物可能会阻止该肽与细胞发生毒性相互作用。
