Sakagami K, Naka H, Hayashi A, Kamei M, Sasabe T, Tano Y
Department of Ophthalmology, Ehime University School of Medicine, Japan.
Ophthalmic Res. 1995;27(5):262-7. doi: 10.1159/000267735.
A new, simple method for isolating retinal pigment epithelial (RPE) cells from rat eyeballs was developed: incubation of the eyeballs in 0.1% proteinase K solution. This method yielded an average of 4 x 10(4) viable RPE cells from each Sprague-Dawley rat eyeball in 1 h. These RPE cells were hexagonal and had cytokeratin in their cytoplasm and numerous microvilli on their surface, similar to RPE cells evaluated in vivo. The results of this study thus show that our method for incubating eyeballs in proteinase K can provide good-quality RPE cells in sufficient quantities for study in a short time.
一种从大鼠眼球中分离视网膜色素上皮（RPE）细胞的新的简单方法被开发出来：将眼球置于0.1%蛋白酶K溶液中孵育。该方法在1小时内从每只斯普拉格-道利大鼠眼球中平均获得4×10⁴个存活的RPE细胞。这些RPE细胞呈六边形，细胞质中有细胞角蛋白，表面有许多微绒毛，类似于体内评估的RPE细胞。因此，本研究结果表明，我们将眼球在蛋白酶K中孵育的方法能够在短时间内提供足够数量的高质量RPE细胞用于研究。
