Inan C, Kiliç I, Kilinç K, Kalayci O, Kotiloğlu E
Pediatr Res. 1995 Nov;38(5):685-9. doi: 10.1203/00006450-199511000-00009.
The protective effects of high dose antenatal vitamin E on hypoxemia in newborn rats were investigated. The subjects were 1-d-old Wistar rats weighing 5-6 g which were born to mothers weighing 245-250 g. Three groups of rat pups, each consisting of eight newborn rats, were used: nontreated control group, hypoxic group, and vitamin E group. The mothers of pups in the last group were given vitamin E (2000 mg/kg/d) antenatally on 3 consecutive days. Hypoxia was induced by breathing of a mixture of 8% oxygen and 92% nitrogen for 3 h. Then pups were allowed to inhale normal atmospheric air for 30 min. All rats were killed on the first day of life after the procedure of hypoxia and reoxygenation. The brains, lungs, livers, intestines, and kidneys were studied biochemically and histopathologically. The hypoxia-induced biochemical changes were determined by measuring lipid peroxidation and myeloperoxidase activity. Vitamin E effectively inhibited hypoxia-induced lipid peroxidation in liver and intestines, and decreased the levels of thiobarbituric acid-reactive substances in brain. In agreement with lipid peroxidation, tissue associated myeloperoxidase activity was increased in liver, intestines, and kidneys, but not in brain and lungs, of the hypoxic group. Histopathologic changes in intestines were epithelial separation and submucosal polymorphonuclear leukocyte infiltration. In the liver, leukocyte infiltration was observed only near the portal areas. These changes were not observed in the vitamin E group. It was concluded that high doses of antenatal vitamin E may protect the newborn rat pups against hypoxia-induced tissue injury.
研究了高剂量产前维生素E对新生大鼠低氧血症的保护作用。研究对象为出生体重5 - 6克的1日龄Wistar大鼠，其母鼠体重为245 - 250克。将大鼠幼崽分为三组，每组八只：未处理对照组、低氧组和维生素E组。最后一组幼崽的母鼠在产前连续3天给予维生素E（2000毫克/千克/天）。通过让大鼠吸入8%氧气和92%氮气的混合气体3小时诱导低氧。然后让幼崽吸入正常大气空气30分钟。在低氧和复氧过程后的出生第一天处死所有大鼠。对大脑、肺、肝脏、肠道和肾脏进行生化和组织病理学研究。通过测量脂质过氧化和髓过氧化物酶活性来确定低氧诱导的生化变化。维生素E有效抑制了肝脏和肠道中低氧诱导的脂质过氧化，并降低了大脑中硫代巴比妥酸反应性物质的水平。与脂质过氧化情况一致，低氧组肝脏、肠道和肾脏中与组织相关的髓过氧化物酶活性增加，但大脑和肺中未增加。肠道的组织病理学变化为上皮分离和黏膜下多形核白细胞浸润。在肝脏中，仅在门静脉区域附近观察到白细胞浸润。在维生素E组中未观察到这些变化。得出的结论是，高剂量产前维生素E可能保护新生大鼠幼崽免受低氧诱导的组织损伤。
