Foster C M, Hashimoto N, Randolph J F, Beitins I Z
Department of Pediatrics, University of Michigan School of Medicine, Ann Arbor 48109, USA.
Pediatr Res. 1995 Nov;38(5):763-7. doi: 10.1203/00006450-199511000-00021.
Growth hormone (GH) appears to affect the timing of puberty in children. The effects of GH on puberty may be related to direct GH action on ovarian function or may be mediated by IGF-I. To determine the likelihood that GH has direct effects on ovarian function, we compared the ability of GH and IGF-I to increase luteinized granulosa cell steroidogenesis in the absence and presence of gonadotropins. Cells were obtained from women undergoing in vitro fertilization for tubal disorders or male factor infertility and were placed in static culture. GH alone failed to alter progesterone or estradiol accumulation in the medium of cultured luteinized granulosa cells. IGF-I produced no increase in progesterone accumulation but increased estradiol accumulation 5.6-fold compared with cells treated with vehicle. The combination of GH and FSH produced an 0.83-fold increase in estradiol accumulation, whereas the combination of IGF-I and FSH resulted in a 2.9-fold increase in estradiol accumulation above FSH alone. Thus the direct effects of GH on granulosa cell steroid synthesis are modest compared with those of IGF-I. If GH has an effect on ovarian development at puberty, it is likely to be mediated by a GH-induced increase in circulating IGF-I.
生长激素（GH）似乎会影响儿童青春期的时间。GH对青春期的影响可能与GH对卵巢功能的直接作用有关，也可能由胰岛素样生长因子-I（IGF-I）介导。为了确定GH对卵巢功能有直接影响的可能性，我们比较了在有无促性腺激素的情况下，GH和IGF-I增加黄素化颗粒细胞类固醇生成的能力。细胞取自因输卵管疾病或男性因素不育而接受体外受精的女性，并进行静态培养。单独使用GH未能改变培养的黄素化颗粒细胞培养基中孕酮或雌二醇的积累。与用赋形剂处理的细胞相比，IGF-I没有增加孕酮的积累，但使雌二醇的积累增加了5.6倍。GH和促卵泡激素（FSH）联合使用使雌二醇的积累增加了0.83倍，而IGF-I和FSH联合使用导致雌二醇的积累比单独使用FSH增加了2.9倍。因此，与IGF-I相比，GH对颗粒细胞类固醇合成的直接影响较小。如果GH在青春期对卵巢发育有影响，很可能是由GH诱导的循环IGF-I增加所介导的。
