通过淀粉凝胶电泳和免疫沉淀联合法对白蛋白制剂中白蛋白二聚体进行定量测定。
Quantitative determination of albumin dimer in albumin preparations by combined starch-gel electrophoresis and immunoprecipitation.
作者信息
Skoog B, Granstrand B
出版信息
Vox Sang. 1977;32(4):242-6. doi: 10.1111/j.1423-0410.1977.tb00637.x.
Abstract
A method is described for albumin dimer determination. The method is based upon an electrophoretic separation of albumin dimer from the monomer in starch gel with a subsequent immunochemical diffusion and precipitation of the albumin components in agarose gel containing antibodies. The precipitation zones are measured and compared iwth those of albumin monomer standards. A factor for converting monomer to dimer has furthermore been established.
摘要
描述了一种测定白蛋白二聚体的方法。该方法基于在淀粉凝胶中通过电泳将白蛋白二聚体与单体分离，随后在含有抗体的琼脂糖凝胶中进行免疫化学扩散和白蛋白成分的沉淀。测量沉淀区并与白蛋白单体标准品的沉淀区进行比较。此外，还建立了将单体转化为二聚体的系数。
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