生物体液中人白蛋白的固相荧光免疫测定法。
Solid-phase fluoroimmunoassay of human albumin in biological fluids.
作者信息
Nargessi R D, Landon J, Pourfarzaneh M, Smith D S
出版信息
Clin Chim Acta. 1978 Nov 1;89(3):455-60. doi: 10.1016/0009-8981(78)90409-6.
A simple fluoroimmunoassay for the determination of albumin levels in serum, urine and cerebrospinal fluid is described. It employs magnetisable particles to which antibodies to human serum albumin are covalently linked, and albumin labelled with fluorescein. Equilibrium is reached within 30 min, when separation of the bound and free fractions of the labelled albumin is performed by precipitation of the particles either with a magnet or by centrifugation. Measurement of the fluorescence in the supernatant (the free fraction) reflects the albumin concentration of the standards or samples. Correlation studies with an automated immunoprecipitation technique show good agreement.
本文描述了一种用于测定血清、尿液和脑脊液中白蛋白水平的简单荧光免疫测定法。该方法使用了共价连接有人血清白蛋白抗体的可磁化颗粒，以及用荧光素标记的白蛋白。30分钟内即可达到平衡，此时通过用磁铁沉淀颗粒或离心来分离标记白蛋白的结合部分和游离部分。测量上清液（游离部分）中的荧光可反映标准品或样品中的白蛋白浓度。与自动免疫沉淀技术的相关性研究显示出良好的一致性。
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