Goldblum J R, Rice T W
Department of Anatomic Pathology, Cleveland Clinic Foundation, Ohio, USA.
Mod Pathol. 1995 Oct;8(8):866-9.
The bcl-2 proto-oncogene encodes a protein that blocks programmed cell death (apoptosis). Although bcl-2 has been shown to be involved in the development of follicular lymphoma via a chromosomal translocation t(14;18), little is known about its function in non-hematolymphoid neoplasms. The bcl-2 protein is normally expressed in the regenerative crypt compartment of the colon, small intestine, and stomach, and has been found to be abnormally overexpressed as an early event in the dysplasia-carcinoma sequences of both ulcerative colitis-related and gastric neoplasias. This study was undertaken to evaluate the role of bcl-2 in the Barrett's metaplasia-dysplasia-carcinoma sequence. Thirty-six esophageal resection specimens were studied, using a monoclonal antibody to the bcl-2 protein on fixed paraffin-embedded specimens. Barrett's mucosa was present in each specimen: low-grade dysplasia in 35, high-grade dysplasia in 34, intramucosal carcinoma (IMC) in 23, and submucosal carcinoma in 13. In addition, a section of the gastric resection margin was evaluated for bcl-2 immunoreactivity in each case. In all cases, the regenerative compartment of the gastric mucosa in the resection margins stained for bcl-2; however, no immunoreactivity was seen in any of the cases of Barrett's mucosa with or without dysplasia or carcinoma. We conclude that, in contrast to its role in gastric neoplasia, bcl-2 alterations are not an important molecular marker in the neoplastic progression of Barrett's mucosa.
bcl-2原癌基因编码一种可阻止程序性细胞死亡（细胞凋亡）的蛋白质。尽管已证实bcl-2通过染色体易位t(14;18)参与滤泡性淋巴瘤的发生发展，但对其在非血液淋巴系统肿瘤中的功能却知之甚少。bcl-2蛋白通常在结肠、小肠和胃的再生隐窝区表达，并且已发现在溃疡性结肠炎相关肿瘤和胃肿瘤的发育异常-癌序列的早期事件中异常过度表达。本研究旨在评估bcl-2在巴雷特化生-发育异常-癌序列中的作用。使用针对固定石蜡包埋标本中bcl-2蛋白的单克隆抗体，对36例食管切除标本进行了研究。每个标本均存在巴雷特黏膜：低级别发育异常35例，高级别发育异常34例，黏膜内癌（IMC）23例，黏膜下癌13例。此外，对每个病例胃切除切缘的一部分进行bcl-2免疫反应性评估。在所有病例中，切除切缘胃黏膜的再生区bcl-2染色阳性；然而，在任何伴有或不伴有发育异常或癌的巴雷特黏膜病例中均未观察到免疫反应性。我们得出结论，与bcl-2在胃肿瘤中的作用相反, bcl-2改变在巴雷特黏膜肿瘤进展中不是一个重要的分子标志物。
