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[酸损伤后金黄色葡萄球菌的恢复]

[Recovery of Staphylococcus aureus after acid damage].

作者信息

Assis E M, de Carvalho E P, Asquieri E R, da Silva F V, Robbs P G

机构信息

Faculdade Engenharia de Alimentos, Departamento de Tecnologia, Universidade Estadual de Campinas, S.P. Brasil.

出版信息

Rev Latinoam Microbiol. 1995 Apr-Jun;37(2):127-34.

PMID:8552872
Abstract

The growth behavior of S. aureus in fresh cheese (Minas and Mozzarella) during their shelf life was studied in this research. The possibility of injury to this microorganism caused by increasing acidity was also investigated. Raw milk was inoculated with S. aureus FRIA-100 with approximately 10(6) cells/ml and cheese production was carried out according to normal procedures. They were stored at 7 degrees C during 40 days for Minas cheese and during 60 days for Mozzarella cheese. At 2 to 3 days intervals the following analyses were performed: acidity, pH, S. aureus count on Baird-Parker agar by traditional methods and by the method recommended by the American Public Health Association, to count repair of injured cells. We were certain of the presence of injured S. aureus when acidity was in the range of 0.7 to 0.8% expressed as lactic acid and when the count was 1.3 log higher.

摘要

本研究对金黄色葡萄球菌在新鲜奶酪(米纳斯奶酪和马苏里拉奶酪)保质期内的生长行为进行了研究。同时还调查了酸度增加对该微生物造成损伤的可能性。将每毫升约含10(6)个细胞的金黄色葡萄球菌FRIA - 100接种到原料乳中,并按照正常程序制作奶酪。米纳斯奶酪在7℃下储存40天,马苏里拉奶酪在7℃下储存60天。每隔2至3天进行以下分析:酸度、pH值、采用传统方法和美国公共卫生协会推荐的方法在贝尔德-帕克琼脂上对金黄色葡萄球菌进行计数,以计算受损细胞的修复情况。当酸度以乳酸计在0.7%至0.8%范围内且菌数高出1.3个对数时,我们确定存在受损的金黄色葡萄球菌。

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