Sallis J D, Parry N F, Meehan J D, Kamperman H, Anderson M E
Department of Biochemistry, University of Tasmania, Hobart, Australia.
Scanning Microsc. 1995 Mar;9(1):127-35; discussion 135-6.
Calcium oxalate (CaOx) crystallization in the presence of phosphocitrate (PC) was studied by both in vitro and in vivo techniques. Crystals of the monohydrate (COM) and the dihydrate (COD) forms were generated under controlled conditions in a silica gel matrix. Our data indicated only COD crystals formed when PC was present, inferring that the COD to COM transformation was being impeded. COD crystals were smaller in size than controls and there was evidence of interpenetral twinning. An in vivo study using a rat bladder implant model noted similar findings. Scanning electron microscopy (SEM) revealed that implants recovered from PC treated rats had primarily COD crystals deposited, whereas both the surface and inner layers of encrusted implants from normal rats contained predominantly COM crystals. Infrared (IR) analysis confirmed the visual findings indicating quantitatively that there was a higher proportion of COD present on the implants recovered from the treated rats than in the controls. It is concluded that although total CaOx crystallization cannot be eliminated by PC, its action could assist in reducing the harmful nature of such crystallites in the urine.
通过体外和体内技术研究了在磷酸柠檬酸（PC）存在下草酸钙（CaOx）的结晶情况。在硅胶基质中，在受控条件下生成了一水合物（COM）和二水合物（COD）形式的晶体。我们的数据表明，当存在PC时，仅形成了COD晶体，这意味着COD向COM的转变受到了阻碍。COD晶体的尺寸比对照小，并且有相互贯穿孪晶的证据。一项使用大鼠膀胱植入模型的体内研究也有类似发现。扫描电子显微镜（SEM）显示，从接受PC治疗的大鼠体内回收的植入物主要沉积有COD晶体，而正常大鼠结壳植入物的表层和内层主要含有COM晶体。红外（IR）分析证实了视觉观察结果，定量表明从接受治疗的大鼠体内回收的植入物上COD的比例高于对照组。得出的结论是，尽管PC不能消除草酸钙的总结晶，但它的作用有助于降低尿液中此类微晶的有害性。
