Suzuki K T, Itoh M, Ohmichi M
Faculty of Pharmaceutical Sciences, Chiba University, Japan.
Toxicology. 1995 Dec 10;103(3):157-65. doi: 10.1016/0300-483x(95)03136-4.
The disposition of selenium (Se) was investigated in Wistar rats of various Se status after an intravenous injection of 82Se-selenite. Various fractions of plasma, urine, and cytosols from liver and kidney were separated by high performance liquid chromatography (HPLC), coupled with an inductively coupled argon plasma-mass spectrometry (ICP-MS). The technique allowed simultaneous differentiation of the fate of injected and endogenous Se, and if it was influenced by the previous Se burden in the tissues. A broad Se-peak from plasma was resolved in two fractions by assessing the m/z 82/78 ratios. Urinary profiles indicated that the metabolism of Se was dose-dependent; monomethylselenol being the primary metabolite of Se in untreated animals, whereas noticeable amount of trimethylselenonium ion was detected after the injection of 82Se. Liver and kidney cytosols contained complex Se-enriched fractions, a positive identification of which was not done in this study. In most cases, the enrichment of tissue fractions with the stable isotope was altered by the dietary Se levels, the isotope nevertheless was exchanged with the endogenous Se in various macromolecules to a varying degree.
在静脉注射82Se-亚硒酸盐后，研究了不同硒状态的Wistar大鼠体内硒（Se）的分布情况。通过高效液相色谱（HPLC）结合电感耦合氩等离子体质谱（ICP-MS），分离了血浆、尿液以及肝脏和肾脏细胞溶质中的不同组分。该技术能够同时区分注入的硒和内源性硒的去向，以及其是否受组织中先前硒负荷的影响。通过评估m/z 82/78比值，血浆中的一个宽硒峰被解析为两个组分。尿液分析表明，硒的代谢呈剂量依赖性；在未处理的动物中，一甲基硒醇是硒的主要代谢产物，而注射82Se后检测到了显著量的三甲基硒离子。肝脏和肾脏细胞溶质中含有复杂的富硒组分，本研究未对其进行明确鉴定。在大多数情况下，稳定同位素在组织组分中的富集情况因膳食硒水平而改变，不过该同位素在不同程度上与各种大分子中的内源性硒发生了交换。
