Dell'Omo G, Wolfer D, Alleva E, Lipp H P
Section of Behavioural Pathophysiology, Istituto Superiore di Sanità, Rome, Italy.
Toxicol Lett. 1995 Nov 15;81(2-3):91-9. doi: 10.1016/0378-4274(95)03412-9.
Following exposure to ozone (O3, 0.6 ppm) from the beginning of neonatal life until weaning, adult CD-1 mice were tested in swimming navigation, a sensitive indicator for hippocampal damage. Control mice received a sham exposure. All mice were tested at 12-13 weeks of age for their ability to find a submerged platform in a fixed location (acquisition: 18 trials, six trials per day) and for capacity to re-orient towards a new platform position (reversal: 12 trials, six trials per day). Exposure to O3 did not produce any significant impairment of swimming navigation during the acquisition phase while it slightly increased the swimming paths during the last day of the reversal phase. Mice exposed to O3 showed a slightly but significantly higher swimming speed during all the days, which was unrelated to differences in body weight and to navigational performances. Moreover, mice exposed to O3 (with the exception of one animal) had a strong tendency to make turns to the left while the controls, independent of sex, preferred clockwise turns. Data are discussed with respect to possible implications with early CNS and immune alteration leading to behavioral asymmetries at adulthood.
从新生期开始直至断奶，成年CD - 1小鼠暴露于臭氧（O3，0.6 ppm）中，之后接受游泳导航测试，这是海马体损伤的一个敏感指标。对照小鼠接受假暴露。所有小鼠在12 - 13周龄时测试其在固定位置找到水下平台的能力（习得：18次试验，每天6次试验）以及重新定位到新平台位置的能力（反转：12次试验，每天6次试验）。在习得阶段，臭氧暴露并未对游泳导航产生任何显著损害，而在反转阶段的最后一天，臭氧暴露略微增加了游泳路径。暴露于臭氧的小鼠在所有天数里游泳速度均略有但显著更高，这与体重差异和导航表现无关。此外，暴露于臭氧的小鼠（除一只动物外）有强烈的向左转弯倾向，而对照小鼠，无论性别，更倾向于顺时针转弯。本文讨论了早期中枢神经系统和免疫改变可能导致成年期行为不对称的相关影响。
