Lebedeva I Iu, Kuz'mina T I, Goĭlo T A
Tsitologiia. 1995;37(3):220-6.
The influence of bovine prolactin (BPL) on DNA synthesis in cultured cow granulosa cells in various media has been studied. BPL exerted a mitogenic effect on granulosa cells of follicles 3-5 mm in diameter, with the effect depending on hormone concentration in the cultural medium. In the medium with bovine growth hormone the maximum effective concentration of BPL was 100 times more than in other used media. The degree of BPL influence on granulosa cell proliferation depended on the degree of cell differentiation. In cultures of granulosa cells taken from follicles 1-2 and 3-5 mm in diameter, while the BPL concentration increased from 500 ng/ml to 5 micrograms/ml in the serum free medium, and from 50 ng/ml to 500 ng/ml in the medium with 5% fetal bovine serum, the stimulation of DNA synthesis in cells decreased. The growth-promoting influence of BPL on granulosa cells of follicles 6-10 mm in diameter did not depend on its concentration in the cultural medium. The maximum mitogenic hormonal effect decreased with the growth of a follicle. This fact is in accord with the recent notions of a progressive reduction of granulosa cell proliferative activity during follicular maturation. The results obtained are discussed in terms of a hypothesis that prolactin can influence granulosa cell secretion of a regulator of meiosis and folliculogenesis, i.e. an inhibitor of oocyte maturation.
研究了牛催乳素（BPL）在各种培养基中对培养的奶牛颗粒细胞DNA合成的影响。BPL对直径3 - 5毫米卵泡的颗粒细胞具有促有丝分裂作用，其作用取决于培养基中激素的浓度。在含有牛生长激素的培养基中，BPL的最大有效浓度比其他所用培养基高100倍。BPL对颗粒细胞增殖的影响程度取决于细胞分化程度。在取自直径1 - 2毫米和3 - 5毫米卵泡的颗粒细胞培养物中，在无血清培养基中，当BPL浓度从500纳克/毫升增加到5微克/毫升时，以及在含有5%胎牛血清的培养基中，当BPL浓度从50纳克/毫升增加到500纳克/毫升时，细胞中DNA合成的刺激作用降低。BPL对直径6 - 10毫米卵泡的颗粒细胞的促生长作用不取决于其在培养基中的浓度。最大促有丝分裂激素效应随卵泡生长而降低。这一事实与最近关于卵泡成熟过程中颗粒细胞增殖活性逐渐降低的观点一致。根据催乳素可影响颗粒细胞分泌减数分裂和卵泡发生调节剂（即卵母细胞成熟抑制剂）的假说，对所得结果进行了讨论。
