Partidos C D, Vohra P, Steward M W
Department of Clinical Sciences, London School of Hygiene and Tropical Medicine, United Kingdom.
Virology. 1996 Jan 1;215(1):107-10. doi: 10.1006/viro.1996.0012.
In this study, the potential of the amino-terminal sequence from the F1 polypeptide responsible for the fusion of measles virus (MV) with cell membranes as a carrier system for a CTL epitope from the MV nucleoprotein was examined. The addition of the fusion sequence (FP) at either the amino or the carboxyl terminus of the CTL epitope peptide rendered it immunogenic after intraperitoneal immunization in mice. The CTLs induced were able to lyse target cells pulsed with the peptide or persistently infected with MV. After intranasal administration of a FP-CTL chimera with or without cholera toxin B subunit (CTB) as an adjuvant, CTL responses to the peptide pulsed and to MV-infected target cells were detected. Responses in groups of mice where CTB was used as an adjuvant were stronger. However, intranasal administration of the CTL epitope did not induce a protective response against intracranial challenge with a neuroadapted strain of MV. These findings highlight the potential of fusion sequences as a carrier system for CTL epitopes and the potential of the intranasal route for administration of synthetic peptides representing MV sequences.
在本研究中，对麻疹病毒（MV）F1多肽负责与细胞膜融合的氨基末端序列作为MV核蛋白CTL表位载体系统的潜力进行了检测。在CTL表位肽的氨基或羧基末端添加融合序列（FP），使其在小鼠腹腔内免疫后具有免疫原性。诱导的CTL能够裂解用该肽脉冲处理或持续感染MV的靶细胞。在鼻内给予含或不含霍乱毒素B亚基（CTB）作为佐剂的FP-CTL嵌合体后，检测到对肽脉冲处理和MV感染靶细胞的CTL反应。使用CTB作为佐剂的小鼠组中的反应更强。然而，鼻内给予CTL表位并未诱导针对神经适应株MV颅内攻击的保护性反应。这些发现突出了融合序列作为CTL表位载体系统的潜力以及鼻内途径用于给予代表MV序列的合成肽的潜力。
