Głazewski S, Kossut M, Skangiel-Kramska J
Department of Neurophysiology, Nencki Institute, Warsaw, Poland.
Int J Dev Neurosci. 1995 Oct;13(6):505-14. doi: 10.1016/0736-5748(95)00051-h.
The development of N-methyl-D-aspartate (NMDA) receptors and the effects of vibrissectomy upon [3H]MK-801 binding were examined in the barrel cortex of mice. Autoradiographic studies showed that initially very low binding of [3H]MK-801 sharply increased during the second postnatal week reaching the adult level by the end of the third week. Scatchard analysis performed on cortical membrane preparations indicated that this rise of [3H]MK-801 labelling was due to an increase in the number of binding sites and a decrease of Kd at postnatal day 15 and 28. The interlaminar differences of labelling were registered from postnatal day 8. Changes of interlaminar distribution were found during the second and third postnatal weeks. In adult barrel cortex the highest binding was found in supragranular layers. In layer IV of the cortex, the pattern of binding resembled the pattern of barrels. Unilateral denervation of vibrissae performed in neonatal and adult mice did not alter the intensity of [3H]MK-801 labelling or the laminar distribution of binding sites. These results suggest that NMDA receptor binding does not reflect the plastic changes occurring in the barrel cortex.
在小鼠桶状皮层中研究了N-甲基-D-天冬氨酸（NMDA）受体的发育以及触须切除对[3H]MK-801结合的影响。放射自显影研究表明，最初[3H]MK-801的结合非常低，在出生后第二周急剧增加，到第三周结束时达到成年水平。对皮层膜制剂进行的Scatchard分析表明，[3H]MK-801标记的这种增加是由于出生后第15天和第28天结合位点数量的增加和Kd的降低。从出生后第8天开始记录标记的层间差异。在出生后第二周和第三周发现了层间分布的变化。在成年桶状皮层中，最高结合出现在颗粒上层。在皮层的第IV层，结合模式类似于桶状模式。在新生和成年小鼠中对触须进行单侧去神经支配不会改变[3H]MK-801标记的强度或结合位点的层状分布。这些结果表明，NMDA受体结合不能反映桶状皮层中发生的可塑性变化。
