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磷脂酶Cδ1需要一个普列克底物蛋白同源结构域来与质膜相互作用。

Phospholipase C delta 1 requires a pleckstrin homology domain for interaction with the plasma membrane.

作者信息

Paterson H F, Savopoulos J W, Perisic O, Cheung R, Ellis M V, Williams R L, Katan M

机构信息

CRC Centre for Cell and Molecular Biology, Chester Beatty Labortories, London, U.K.

出版信息

Biochem J. 1995 Dec 15;312 ( Pt 3)(Pt 3):661-6. doi: 10.1042/bj3120661.

DOI:10.1042/bj3120661
PMID:8554502
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1136164/
Abstract

The structural requirements of phospholipase C delta 1 for interaction with the plasma membrane were analysed by immunofluorescence after microinjection into living cells. Microinjection of deletion mutants revealed that the region required for membrane attachment and binding of inositol 1,4,5-trisphosphate in vitro corresponded to the pleckstrin homology domain, a structural module described in more than 90 proteins.

摘要

通过显微注射到活细胞中后进行免疫荧光分析，研究了磷脂酶Cδ1与质膜相互作用的结构要求。对缺失突变体的显微注射显示，体外膜附着和与肌醇1,4,5-三磷酸结合所需的区域对应于普列克底物蛋白同源结构域，这是一种在90多种蛋白质中描述过的结构模块。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1da7/1136164/8cb06fe22c93/biochemj00049-0026-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1da7/1136164/ddb476865075/biochemj00049-0023-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1da7/1136164/75a1d6f9ca81/biochemj00049-0024-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1da7/1136164/613170042b3b/biochemj00049-0025-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1da7/1136164/8cb06fe22c93/biochemj00049-0026-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1da7/1136164/ddb476865075/biochemj00049-0023-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1da7/1136164/75a1d6f9ca81/biochemj00049-0024-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1da7/1136164/613170042b3b/biochemj00049-0025-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1da7/1136164/8cb06fe22c93/biochemj00049-0026-a.jpg

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Phospholipase C-gamma 1 associates with viral and cellular src kinases.磷脂酶C-γ1与病毒和细胞源激酶相关联。
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