Sconzo G, Ferraro M G, Amore G, Giudice G, Cascino D, Scardina G
Dipartimento di Biologia Cellulare e dello Sviluppo Alberto Monroy, Viale delle Scienze, Università di Palermo, Italy.
Biochem Biophys Res Commun. 1995 Dec 26;217(3):1032-8. doi: 10.1006/bbrc.1995.2873.
We had previously shown that P. lividus embryos subjected to heat shock are unable to synthesize proteins of the hsp70 family at a detectable level before the hatching blastula stage. We show here that this is not due to the inability to synthesize the hsp70 mRNAs, which are detectable following heat shock also in early stages, although in much lower amounts per embryo than in later stages. These mRNAs are also translated, as judged by the facts that they are present in the polysomal pellet, and that they are translatable in a cell free system. As to the question of the amount of hsp70 RNAs per nucleus, we conclude that this is also higher in later than in earlier stages. The presence of hsp70 mRNAs is already detectable after heating at 4 centigrades above 20 and their amount increases with the increase of temperature in the range between 24 degrees C and 28 degrees C.
我们之前已经表明，遭受热休克的紫海胆胚胎在孵化囊胚阶段之前无法在可检测水平合成热休克蛋白70（hsp70）家族的蛋白质。我们在此表明，这并非由于无法合成hsp70信使核糖核酸（mRNAs），热休克后在早期阶段也可检测到这些信使核糖核酸，尽管每个胚胎中的含量比后期阶段低得多。这些信使核糖核酸也会被翻译，这可从它们存在于多核糖体沉淀中以及它们在无细胞系统中可被翻译这两个事实判断得出。关于每个细胞核中hsp70核糖核酸（RNAs）的量的问题，我们得出结论，后期阶段的量也高于早期阶段。在高于20摄氏度4摄氏度加热后即可检测到hsp70信使核糖核酸的存在，并且它们的量在24摄氏度至28摄氏度的温度范围内随温度升高而增加。
