Kawamura N, Imanishi N, Koike H, Nakahara H, Phillips L, Morooka S
Sumitomo Pharmaceuticals, Research Center, Osaka, Japan.
Biochem Biophys Res Commun. 1995 Dec 26;217(3):1208-15. doi: 10.1006/bbrc.1995.2897.
We investigated whether lipoteichoic acid (LTA) induces the expression of E-selectin and neutrophil adhesion to human umbilical vein endothelial cells (HUVECs). LTA (0.01-30 micrograms/ml) induced the expression of E-selectin in a concentration-dependent manner with maximal response at 10 micrograms/ml. The expression level of E-selectin increased from 2 h after stimulation by LTA with the maximal level at 4-8 h. Neutrophil adhesion to LTA-treated HUVECs correlated with the levels of E-selectin expression. In addition, the adhesion was clearly inhibited by anti-human E-selectin monoclonal antibody CY-1787 as well as a sialyl Lewis X (SLeX) oligosaccharide. LTA-induced expression of E-selectin was inhibited by protein kinase C inhibitors, H-7 and staurosporine. These results indicate that LTA induced the expression of E-selectin and neutrophils adhered to HUVECs via E-selectin.
我们研究了脂磷壁酸（LTA）是否诱导E-选择素的表达以及中性粒细胞与人脐静脉内皮细胞（HUVECs）的黏附。LTA（0.01 - 30微克/毫升）以浓度依赖的方式诱导E-选择素的表达，在10微克/毫升时达到最大反应。E-选择素的表达水平在LTA刺激后2小时开始增加，在4 - 8小时达到最高水平。中性粒细胞与经LTA处理的HUVECs的黏附与E-选择素的表达水平相关。此外，抗人E-选择素单克隆抗体CY - 1787以及唾液酸化路易斯X（SLeX）寡糖可明显抑制这种黏附。LTA诱导的E-选择素表达受到蛋白激酶C抑制剂H - 7和星形孢菌素的抑制。这些结果表明，LTA诱导E-选择素的表达，并且中性粒细胞通过E-选择素黏附于HUVECs。
