在小鼠骨髓性白血病NFS60细胞中，所谓的“a”和“去唾液酸”途径糖鞘脂的生物合成受到不同的调控。
Biosynthesis of the so-called "a" and "asialo" pathway glycosphingolipids is differentially regulated in murine myelogenous leukemia NFS60 cells.
作者信息
Nakamura M, Sakai T, Furukawa Y, Kitagawa S, Sakoe K, Tsunoda A
机构信息
Division of Hemopoiesis, Institute of Hematology, Jichi Medical School, Tochigi, Japan.
出版信息
Biochem Biophys Res Commun. 1995 Dec 26;217(3):733-40. doi: 10.1006/bbrc.1995.2834.
Regulation of "a" and "asialo" series ganglioside biosynthesis was analyzed. COS-1 cells expressing only GD1a showed high synthetic activities of GM3, GM2, GM1a, and GD1a, but little activity for GA2 synthesis. However, IL-3-dependent murine NFS60-I7, which has GM1b and GD1 alpha, exhibited high synthetic activities of GM2, GM1a, and GD1a, but GM3 synthase was only 1/6 of COS-1 and GA2 synthetic activity was low. By contrast, IL-3 gene-transfected subline NFS60-H7 expressing GD1a in addition to GM1b and GD1 alpha displayed up-regulated GM3 synthase and GA2 synthase activities, while GM2, GM1a, and GD1a synthase activities were in the same levels as in NFS60-I7 cells. Since GA2 synthetic activities were not parallel with GM2 synthase in the investigated machinery of ganglioside biosynthesis, it is strongly suggested that biosynthesis of "a" and "asialo" series gangliosides is regulated differentially from each other in murine NFS60 cell lines.
对“a”系列和“去唾液酸”系列神经节苷脂生物合成的调控进行了分析。仅表达GD1a的COS - 1细胞显示出GM3、GM2、GM1a和GD1a的高合成活性，但GA2合成活性很低。然而，具有GM1b和GD1α的依赖白细胞介素-3的小鼠NFS60 - I7表现出GM2、GM1a和GD1a的高合成活性，但GM3合酶仅为COS - 1的1/6，且GA2合成活性较低。相比之下，除了GM1b和GD1α之外还表达GD1a的白细胞介素-3基因转染亚系NFS60 - H7显示出上调的GM3合酶和GA2合酶活性，而GM2、GM1a和GD1a合酶活性与NFS60 - I7细胞中的水平相同。由于在所研究的神经节苷脂生物合成机制中GA2合成活性与GM2合酶不平行，强烈表明在小鼠NFS60细胞系中“a”系列和“去唾液酸”系列神经节苷脂的生物合成相互之间受到不同的调控。
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