Ott D E, Coren L V, Johnson D G, Sowder R C, Arthur L O, Henderson L E
AIDS Vaccine Program, National Cancer Institute, Frederick Cancer Research and Development Center, Maryland 21702-1201, USA.
AIDS Res Hum Retroviruses. 1995 Sep;11(9):1003-6. doi: 10.1089/aid.1995.11.1003.
Previous reports have shown that cyclophilin A (CyPA) is found to be specifically associated with human immunodeficiency virus type-1 (HIV-1) virions and is required for infectivity (Franke et al. Nature 372:359; Thali et al. Nature 372:363). We have examined CyPA associated with HIV-1MN virions. Virions from infected human lymphoid cells were analyzed by high-pressure liquid chromatography (HPLC), protein sequence, and immunoblot analysis. At least three forms of CyPA were found: an unmodified form, an N-terminally modified form, and an N-terminally modified form that migrates as a larger isoform on a reducing-SDS polyacrylamide gel. Using a protease digestion procedure, CyPA that is associated with virions was found to be located inside the viral membrane. Similar examination of SIVMne produced by HUT-78 human T cells did not detect specific incorporation of CyPA into SIV virions. Our results are consistent with the role of CyPA acting early in the infectious process of HIV-1.
先前的报告显示，亲环素A（CyPA）被发现与1型人类免疫缺陷病毒（HIV-1）病毒粒子特异性相关，并且是感染性所必需的（弗兰克等人，《自然》372:359；塔利等人，《自然》372:363）。我们已经检测了与HIV-1MN病毒粒子相关的CyPA。通过高压液相色谱（HPLC）、蛋白质序列分析和免疫印迹分析对来自受感染人类淋巴细胞的病毒粒子进行了分析。发现了至少三种形式的CyPA：未修饰形式、N端修饰形式以及在还原SDS聚丙烯酰胺凝胶上迁移为较大异构体的N端修饰形式。使用蛋白酶消化程序，发现与病毒粒子相关的CyPA位于病毒膜内部。对由HUT-78人T细胞产生的SIVMne进行的类似检测未检测到CyPA特异性掺入SIV病毒粒子。我们的结果与CyPA在HIV-1感染过程早期发挥作用的观点一致。
