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[用于霍乱诊断的干碱性琼脂生产用蛋白胨的理化性质比较研究]

[Comparative study of the physico-chemical properties of peptones used to the production of dry alkaline agar for cholera diagnosis].

作者信息

Artemenko V D, Nenashev V P, Baranova T N, Tokinova T N, Riapolova R G

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1977 Feb(2):125-9.

PMID:855517
Abstract

The authors present the results of chemical and physico-chemical study of dry peptones (made in the USSR) used in the production of dry alkaline nutrient agar for the diagnosis of cholera, in comparison with peptones used for other purposes. The batches and types of peptones studied differed by the fractional peptide composition and by the physico-chemical characteristics. Positive results were obtained in the biological test in using peptones with marked hydrolysis with the amine nitrogen content of not less than 200--230 mg%, with an elution curve approaching the Hottinger's peptone curve, well soluble in buffer phosphate solution at 5% concentration.

摘要

作者介绍了用于霍乱诊断的干燥碱性营养琼脂生产中使用的干蛋白胨(苏联生产)的化学和物理化学研究结果,并与用于其他目的的蛋白胨进行了比较。所研究的蛋白胨批次和类型在分级肽组成和物理化学特性方面存在差异。在生物测试中,使用具有明显水解作用、胺氮含量不低于200-230毫克%、洗脱曲线接近霍廷格蛋白胨曲线且在5%浓度的磷酸盐缓冲溶液中溶解性良好的蛋白胨,获得了阳性结果。

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