Park N G, Yamato Y, Lee S, Sugihara G
Department of Biotechnology and Bioengineering, College of Fisheries Science, National Fisheries, University of Pusan, Korea.
Biopolymers. 1995 Dec;36(6):793-801. doi: 10.1002/bip.360360611.
Mastoparan B (MP-B), an amphiphilic alpha-helical peptide newly isolated from the hornet Vespa basalis, was studied in comparison with mastoparan (MP), in terms of interaction with the phospholipid bilayer and of hemolytic and antimicrobial activity. The amphiphilic structure of MP-B has more hydrophilic amino acid residues in the hydrophilic surface than that of MP. Although each peptide had a considerably different effect on the interaction with lipid bilayers (e.g., their conformation in the presence of acidic and of neutral lipids and dye-release ability from the encapsulated liposomes), on the whole the interaction mode was similar. MP-B caused a change in the shape of erythrocytes from normal discoid to a crenated form (named echinocytes). MP exhibited strong activity against gram-positive bacteria but not against gram-negative ones. Contrary to this, MP-B showed both strong activity against gram-positive bacteria and potent activity against gram-negative bacteria. Whereas both peptides have almost the same residues on the hydrophobic side, the difference in the hydrophilic surface area on the molecules seems to lead to the subtle change in its interaction with membranes, resulting in the alteration of biological activity.
蜂毒肽B（MP - B）是一种新从黄蜂基胡蜂（Vespa basalis）中分离出的两亲性α - 螺旋肽，本研究将其与蜂毒肽（MP）在与磷脂双层的相互作用、溶血活性和抗菌活性方面进行了比较。MP - B的两亲性结构在亲水表面比MP具有更多的亲水氨基酸残基。尽管每种肽对与脂质双层的相互作用有相当不同的影响（例如，它们在酸性和中性脂质存在下的构象以及从包封脂质体中释放染料的能力），但总体而言相互作用模式相似。MP - B导致红细胞形状从正常盘状变为锯齿状（称为棘状红细胞）。MP对革兰氏阳性菌表现出强活性，但对革兰氏阴性菌无活性。与此相反，MP - B对革兰氏阳性菌和革兰氏阴性菌均表现出强活性。虽然两种肽在疏水侧具有几乎相同的残基，但分子亲水表面积的差异似乎导致其与膜相互作用的细微变化，从而导致生物活性的改变。
