Cerovský Václav, Slaninová Jirina, Fucík Vladimír, Hulacová Hana, Borovicková Lenka, Jezek Rudolf, Bednárová Lucie
Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Flemingovo nám. 2, 166 10 Prague 6, Czech Republic.
Peptides. 2008 Jun;29(6):992-1003. doi: 10.1016/j.peptides.2008.02.007. Epub 2008 Feb 19.
Four new peptides of the mastoparan family, characterized recently in the venom of three neotropical social wasps collected in the Dominican Republic, Polistes major major, Polistes dorsalis dorsalis and Mischocyttarus phthisicus were synthesized and tested for antimicrobial potency against Bacillus subtilis, Staphylococcus aureus, Escherichia coli (E.c.) and Pseudomonas aeruginosa, and for hemolytic and mast cells degranulation activities. As these peptides possess strong antimicrobial activity (minimal inhibitory concentration (MIC) values against Bacillus subtillis and E.c. in the range of 5-40 microM), we prepared 40 of their analogs to correlate biological activities, especially antimicrobial, with the net positive charge, hydrophobicity, amphipathicity, peptide length, amino acid substitutions at different positions of the peptide chain, N-terminal acylation and C-terminal deamidation. Circular dichroism spectra of the peptides measured in the presence of trifluoroethanol or SDS showed that the peptides might adopt alpha-helical conformation in such anisotropic environments.
最近在多米尼加共和国采集的三种新热带社会性黄蜂（大胡蜂、背胡蜂和细腹胡蜂）的毒液中鉴定出了四种新的马斯托帕兰家族肽段，对其进行了合成，并测试了它们对枯草芽孢杆菌、金黄色葡萄球菌、大肠杆菌和铜绿假单胞菌的抗菌效力，以及溶血和肥大细胞脱粒活性。由于这些肽具有很强的抗菌活性（对枯草芽孢杆菌和大肠杆菌的最低抑菌浓度（MIC）值在5-40微摩尔范围内），我们制备了40种类似物，以将生物活性，特别是抗菌活性，与净正电荷、疏水性、两亲性、肽长度、肽链不同位置的氨基酸取代、N端酰化和C端脱酰胺作用联系起来。在三氟乙醇或十二烷基硫酸钠存在下测量的肽的圆二色光谱表明，这些肽在这种各向异性环境中可能会采用α-螺旋构象。
