Vancetto M D, Curi L C, Pereira C A
Instituto Butantan, São Paulo, Brasil.
Braz J Med Biol Res. 1995 May;28(5):553-6.
We determined the ability of a mixture of gangliosides (16% GD1b, 19% GT1b, 21% GM1, 40% GD1a) to neutralize the effect of Crotalus durissus terrificus (Cdt) venom in vitro and in vivo. Protection was indicated by the absence of muscular contractions, hind limb paralysis or death of BALB/c mice (16-18 g) after receiving Cdt venom (1 microgram Cdt venom containing 0.6 microgram protein) at the doses indicated. A dose of Cdt venom above 0.9 microgram (ip) or 1 microgram (im) induced muscular contraction and above 1.2 micrograms (ip) or 5.5 micrograms (im) the venom induced muscular contraction and hind limb paralysis. Cdt venom above 2.5 micrograms (ip) or 9 micrograms (im) induced all these symptoms and 95 to 100% death in experimental animals. The lethal dose 50% of the Cdt venom used was 8 micrograms (im) and 1.5 micrograms (ip). In in vitro studies, 4 mg gangliosides neutralized the effect of up to 1.5 micrograms Cdt venom. Quantities as low as 0.2 mg gangliosides were capable of neutralizing 0.9 microgram of Cdt venom in vitro. Intramuscular treatment with 1 mg gangliosides performed 60 min after the intramuscular injection of 5 micrograms Cdt venom protected 100% of the animals. In contrast, no protection was achieved with intraperitoneal treatment with gangliosides. The data show that gangliosides were effective in neutralizing the toxic effects induced by Crotalus durissus terrificus venom both in vitro and in vivo and that post-exposure intramuscular treatment with gangliosides could protect animals experimentally inoculated with the venom.
我们测定了神经节苷脂混合物（16% GD1b、19% GT1b、21% GM1、40% GD1a）在体外和体内中和南美巨蝮（Cdt）毒液作用的能力。在给予指定剂量的Cdt毒液（1微克含0.6微克蛋白质的Cdt毒液）后，BALB/c小鼠（16 - 18克）未出现肌肉收缩、后肢麻痹或死亡，即表明有保护作用。剂量高于0.9微克（腹腔注射）或1微克（肌肉注射）的Cdt毒液会引起肌肉收缩，高于1.2微克（腹腔注射）或5.5微克（肌肉注射）时，毒液会引起肌肉收缩和后肢麻痹。剂量高于2.5微克（腹腔注射）或9微克（肌肉注射）的Cdt毒液会引发所有这些症状，并导致实验动物95%至百分之百死亡。所用Cdt毒液的半数致死量为8微克（肌肉注射）和1.5微克（腹腔注射）。在体外研究中，4毫克神经节苷脂可中和高达1.5微克Cdt毒液的作用。低至0.2毫克的神经节苷脂在体外就能中和0.9微克的Cdt毒液。在肌肉注射5微克Cdt毒液60分钟后，用1毫克神经节苷脂进行肌肉注射治疗，可使100%的动物得到保护。相比之下，神经节苷脂腹腔注射治疗未实现保护作用。数据表明，神经节苷脂在体外和体内均能有效中和南美巨蝮毒液诱导的毒性作用，且毒液暴露后用神经节苷脂进行肌肉注射治疗可保护经实验接种该毒液的动物。
