The synthesis and compositional analysis of phosphopeptides.

Protein phosphorylation plays a critical role in the regulation of cell growth and differentiation. There is considerable interest, therefore, in the facile synthesis of peptides that possess selectively phosphorylated residues for use as molecular probes in mechanistic studies of the biological consequences of phosphorylation. This work will review the various synthetic protocols used in the generation of phosphopeptides and will discuss their characterization by amino acid compositional analysis.