Leforestier A, Nissen H U, Dubochet J
Laboratoire de physique des solides, CNRS URA 002, Université Paris-Sud, Orsay, France.
C R Acad Sci III. 1995 Oct;318(10):1015-20.
Thin vitrified films of 146 base pair double-stranded DNA fragments in semi-dilute solutions are observed by cryo-electron microscopy. Depending on the salt conditions of the buffer and the DNA concentration, the molecules form different characteristic arrangements: they are arranged either locally parallel to each other in random overall orientation or packed perpendicular to the film surface, or else are randomly oriented. These arrangements reflect the weak forces acting between the DNA molecules themselves, as well as between the molecules and the thin film surface.
通过冷冻电子显微镜观察半稀释溶液中146个碱基对双链DNA片段的薄玻璃化膜。根据缓冲液的盐条件和DNA浓度，分子形成不同的特征排列：它们要么在整体随机取向下局部彼此平行排列，要么垂直于膜表面堆积，要么随机取向。这些排列反映了DNA分子自身之间以及分子与薄膜表面之间作用的弱力。
