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通过免疫印迹分析进行人单核细胞埃立克体病的血清学诊断。

Serologic diagnosis of human monocytic ehrlichiosis by immunoblot analysis.

作者信息

Brouqui P, Lecam C, Olson J, Raoult D

机构信息

Unité des Rickettsies, Faculté de Médecine, Marseilles, France.

出版信息

Clin Diagn Lab Immunol. 1994 Nov;1(6):645-9. doi: 10.1128/cdli.1.6.645-649.1994.

DOI:10.1128/cdli.1.6.645-649.1994
PMID:8556515
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC368382/
Abstract

Human monocytic ehrlichiosis is caused by Ehrlichia chaffeensis, an intracellular bacterium probably transmitted by the tick Amblyomma americanum in the United States. Despite its lack of specificity in discriminating among infections by closely related Ehrlichia spp., immunofluorescence assay (IFA) is the most frequently used serological diagnostic method. To improve the specificity of the serological diagnosis, we compared antigenic profile of E. canis and E. chaffeensis antigen with homologous and heterologous sera, searching for the specificity of the presence of low-molecular-weight proteins. Western immunoblot analysis of IFA-positive human sera revealed 27- and 29-kDa proteins which are not found in E. canis IFA-positive sera from dogs. IFA-positive sera from dogs revealed a low-molecular-weight group of proteins (20 to 28 kDa) which were not found in human E. chaffeensis-positive sera except for a weak band at 22 kDa. The presence o antibodies directed against the 27- and 29-kDa proteins on Western blots is specific for E. chaffeensis infection, and we suggest that the Western blot might complete IFA in cases with low positive predictive value.

摘要

人单核细胞埃立克体病由恰菲埃立克体引起，恰菲埃立克体是一种细胞内细菌，在美国可能由美洲钝眼蜱传播。尽管免疫荧光测定法（IFA）在区分密切相关的埃立克体属感染方面缺乏特异性，但它仍是最常用的血清学诊断方法。为提高血清学诊断的特异性，我们用同源和异源血清比较了犬埃立克体和恰菲埃立克体抗原的抗原谱，寻找低分子量蛋白存在的特异性。对IFA阳性的人血清进行的Western免疫印迹分析显示出27 kDa和29 kDa的蛋白，而在犬的犬埃立克体IFA阳性血清中未发现这些蛋白。犬的IFA阳性血清显示出一组低分子量蛋白（20至28 kDa），除了在22 kDa处有一条弱带外，在人恰菲埃立克体阳性血清中未发现这些蛋白。Western印迹上针对27 kDa和29 kDa蛋白的抗体的存在对恰菲埃立克体感染具有特异性，我们建议在阳性预测值较低的情况下，Western印迹可作为IFA的补充。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/48d2/368382/fdfc18be1376/cdli00006-0048-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/48d2/368382/971f3c02c04b/cdli00006-0048-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/48d2/368382/fdfc18be1376/cdli00006-0048-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/48d2/368382/971f3c02c04b/cdli00006-0048-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/48d2/368382/fdfc18be1376/cdli00006-0048-b.jpg

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本文引用的文献

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Identification of the antigenic constituents of Ehrlichia chaffeensis.恰菲埃立克体抗原成分的鉴定
Am J Trop Med Hyg. 1994 Jan;50(1):52-8.
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Human ehrlichiosis in New England.新英格兰地区的人粒细胞埃立克体病。
N Engl J Med. 1994 Jan 27;330(4):292-3. doi: 10.1056/NEJM199401273300418.
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Ehrlichia chaffeensis and Rochalimaea antibodies in Kawasaki disease.川崎病中的查菲埃立克体和罗卡利马氏体抗体。
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Detection of antibodies to Anaplasma phagocytophilum and Ehrlichia chaffeensis antigens in sera of Korean patients by western immunoblotting and indirect immunofluorescence assays.通过蛋白质免疫印迹法和间接免疫荧光法检测韩国患者血清中嗜吞噬细胞无形体和查菲埃立克体抗原的抗体
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Serologic and molecular detection of Ehrlichia chaffeensis and Anaplasma phagocytophila (human granulocytic ehrlichiosis agent) in Korean patients.韩国患者中查菲埃立克体和嗜吞噬细胞无形体（人粒细胞埃立克体病病原体）的血清学及分子检测
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Clin Diagn Lab Immunol. 1997 Nov;4(6):731-5. doi: 10.1128/cdli.4.6.731-735.1997.
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Identification of a granulocytotropic Ehrlichia species as the etiologic agent of human disease.鉴定一种嗜粒细胞埃立克体菌种为人类疾病的病原体。
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