Jeong H G, Yang K H
Department of Environmental Science, Chosun University, Dong-ku, Kwangju City, South Korea.
Cancer Lett. 1996 Jan 2;98(2):193-8.
The induction of cytochrome P4501A1 (P4501A1) and P4501A1-specific 7-ethoxyresorufin O-deethylase (EROD) activity by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) was investigated in human splenic lymphocytes cultures. EROD activity was induced by TCDD in mitogen (phytohemagglutinin and pokeweed mitogen) stimulated blast cells but not in the resting cells. TCDD markedly induced EROD activity in a dose- and time-dependent manner. The expression of P4501A1 mRNA was increased by TCDD in mitogen-stimulated cells as detected by Northern blot analysis. These findings support the conclusion that TCDD induced the expression of P4501A1 gene, resulting in increased EROD activity in mitogen-stimulated human splenic lymphocytes cultures.
在人脾淋巴细胞培养物中研究了2,3,7,8-四氯二苯并对二恶英（TCDD）对细胞色素P4501A1（P4501A1）和P4501A1特异性7-乙氧基异吩恶唑酮O-脱乙基酶（EROD）活性的诱导作用。TCDD在有丝分裂原（植物血凝素和商陆有丝分裂原）刺激的母细胞中诱导了EROD活性，但在静止细胞中未诱导。TCDD以剂量和时间依赖性方式显著诱导EROD活性。通过Northern印迹分析检测到，TCDD在有丝分裂原刺激的细胞中增加了P4501A1 mRNA的表达。这些发现支持以下结论：TCDD诱导了P4501A1基因的表达，导致有丝分裂原刺激的人脾淋巴细胞培养物中EROD活性增加。
