Zhang L, Zhan B, Wang J, Feng X
Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1995;13(3):203-8.
Genomic DNA from a Plasmodium falciparum isolate (CMP-1) collected from a falciparum malaria patient at Mengpeng Township, Mengla County, Yunnan Province was used as the template for PCR amplification primered with oligonucleotides from the conserved regions of the apical membrane antigen I (AMA-1) as reported. PCR products recovered from the low melting-point agarose gel electrophoresis were digested with BamHI and EcoRI to generate fragments approximately 420, 445 and 900 bp which were used as the inserts to insert into the M13 mp8 and M13 mp9 vectors for sequencing using 373 sequencer. 1773 bases were read out, among which 17 point mutations were found to result in substitutions of 15 codens which were non-synonymous mutations except for one as compared with that of the consensus. In particular, a relatively hot region of variation was apparent between amino acids 160 and 210.
从云南省勐腊县勐捧镇一名恶性疟患者身上采集的恶性疟原虫分离株（CMP-1）的基因组DNA，用作PCR扩增的模板，PCR扩增使用如报道的来自顶端膜抗原I（AMA-1）保守区域的寡核苷酸作为引物。从低熔点琼脂糖凝胶电泳回收的PCR产物用BamHI和EcoRI消化，产生约420、445和900 bp的片段，这些片段用作插入片段，插入到M13 mp8和M13 mp9载体中，使用373测序仪进行测序。读出了1773个碱基，其中发现17个点突变导致15个密码子的替换，与共有序列相比，除了一个之外，这些都是非同义突变。特别是，在氨基酸160和210之间明显存在一个相对高变异的区域。
